DNA Aptamers are functional molecular recognition sensors in protic Ionic liquids

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dc.contributor.authorMachado, Isabel
dc.contributor.authorÖzalp, Veli Cengiz
dc.contributor.authorRezabal, Elixabete
dc.contributor.authorSchaefer, Thomas
dc.date.accessioned2021-05-15T12:40:42Z
dc.date.available2021-05-15T12:40:42Z
dc.date.issued2014
dc.departmentTıp Fakültesi, Moleküler Kimya Anabilim Dalıen_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990; ASTIGARRAGA, ELIXABETE REZABAL/0000-0003-0397-6140
dc.description.abstractThe function and structural changes of an AMP molecular aptamer beacon and its molecular recognition capacity for its target, adenosine monophosphate (AMP), was systematically explored in solution with a protic ionic liquid, ethylammonium nitrate (EAN). It could be proven that up to 2 M of EAN in TBS buffer, the AMP molecular aptamer beacon was still capable of recognizing AMP while also maintaining its specificity. The specificity was proven by using the guanosine monophosphate (GMP) as target; GMP is structurally similar to AMP but was not recognized by the aptamer. We also found that in highly concentrated EAN solutions the overall amount of double stranded DNA formed, as well as its respective thermal stability, diminished gradually, but surprisingly the hybridization rate (k(h)) of single stranded DNA was significantly accelerated in the presence of EAN. The latter may have important implications in DNA technology for the design of biosensing and DNA-based nanodevices in nonconventional solvents, such as ionic liquids.en_US
dc.description.sponsorshipERCEuropean Research Council (ERC)European Commission [209842 - Matrix]en_US
dc.description.sponsorshipThis work was supported by the ERC Starting Grant 209842 - Matrix. T.S. gratefully acknowledges being hosted in the DIPC, Donostia-San Sebastian. Technical and human support provided by IZO-SGI, SGIker (UPV/EHU, MICINN, GV/EJ, ERDF and ESF) is gratefully acknowledged.en_US
dc.identifier.doi10.1002/chem.201403354
dc.identifier.endpage11825en_US
dc.identifier.issn0947-6539
dc.identifier.issn1521-3765
dc.identifier.issue37en_US
dc.identifier.pmid25065686
dc.identifier.scopus2-s2.0-84906951055
dc.identifier.scopusqualityQ1
dc.identifier.startpage11820en_US
dc.identifier.urihttps://doi.org/10.1002/chem.201403354
dc.identifier.urihttps://hdl.handle.net/20.500.12939/659
dc.identifier.volume20en_US
dc.identifier.wosWOS:000341630500030
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzalp, Veli Cengiz
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofChemistry-A European Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAptamersen_US
dc.subjectDNAen_US
dc.subjectIonic Liquidsen_US
dc.subjectMolecular Recognitionen_US
dc.titleDNA Aptamers are functional molecular recognition sensors in protic Ionic liquids
dc.typeArticle

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