Differentiation potential and tumorigenic risk of rat Bone Marrow stem cells are affected by long-term in vitro expansion

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dc.contributor.authorKaraöz, Erdal
dc.contributor.authorTepeköy, Filiz
dc.date.accessioned2021-05-15T12:41:20Z
dc.date.available2021-05-15T12:41:20Z
dc.date.issued2019
dc.departmentTıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalıen_US
dc.descriptionTepekoy, Filiz/0000-0003-1901-3787
dc.description.abstractObjective: Mesenchymal stem cells (MSCs) have the capacity for extensive expansion and adipogenic, osteogenic, chondrogenic, myogenic, and neural differentiation in vitro. The aim of our study was to determine sternness, differentiation potential, telomerase activity, and ultrastructural characteristics of long-term cultured rat bone marrow (rBM)-MSCs. Materials and Methods: rBM-MSCs from passages 3, 50, and 100 (P3, P50, and P100) were evaluated through immunocytochemistry, reverse transcription-polymerase chain reaction, telomerase activity assays, and electron microscopy. Results: A dramatic reduction in the levels of myogenic markers actin and myogenin was detected in P100. Osteogenic markers Coln, osteonectin (Sparc), and osteocalcin as well as neural marker c-Fos and chondmgenic marker Coll2 were significantly reduced in P100 compared to P3 and P50. Osteogenic marker bone morphogenic protein-2 (BMP2) and adipogenic marker peroxisome proliferator-activated receptor gamma (Ppary) expression was reduced in late passages. The expression of sternness factor Rex-1 was lower in P100, whereas Oct4 expression was decreased in P50 compared to P3 and P100. Increased telomerase activity was observed in long-term cultured cells, signifying tumorigenic risk. Electron microscopic evaluations revealed ultrastructural changes such as smaller number of organelles and increased amount of autophagic vacuoles in the cytoplasm in long-term cultured rBM-MSCs. Conclusion: This study suggests that long-term culture of rBM-MSCs leads to changes in differentiation potential and increased tumorigenic risk.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Kocaeli UniversityKocaeli Universityen_US
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Coordination Unit of Kocaeli University.en_US
dc.identifier.doi10.4274/tjh.galenos.2019.2019.0100
dc.identifier.endpage265en_US
dc.identifier.issn1300-7777
dc.identifier.issn1308-5263
dc.identifier.issue4en_US
dc.identifier.pmid31284704
dc.identifier.scopus2-s2.0-85075101460
dc.identifier.scopusqualityQ3
dc.identifier.startpage255en_US
dc.identifier.urihttps://doi.org/10.4274/tjh.galenos.2019.2019.0100
dc.identifier.urihttps://hdl.handle.net/20.500.12939/787
dc.identifier.volume36en_US
dc.identifier.wosWOS:000497303100006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTepeköy, Filiz
dc.language.isoen
dc.publisherGalenos Yayinciliken_US
dc.relation.ispartofTurkish Journal of Hematology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBone Marrowen_US
dc.subjectDifferentiationen_US
dc.subjectLong-Term Cultureen_US
dc.subjectMesenchymal Stromal Cellsen_US
dc.subjectSternnessen_US
dc.subjectTelomeraseen_US
dc.titleDifferentiation potential and tumorigenic risk of rat Bone Marrow stem cells are affected by long-term in vitro expansion
dc.typeArticle

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