Genotyping of single nucleotide polymorphism by probe-gated silica nanoparticles

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dc.contributor.authorErcan, Meltem
dc.contributor.authorÖzalp, Veli C.
dc.contributor.authorTuna, Bilge G.
dc.date.accessioned2021-05-15T12:42:19Z
dc.date.available2021-05-15T12:42:19Z
dc.date.issued2017
dc.departmentTıp Fakültesien_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990; Tuna, Bilge Guvenc/0000-0003-1348-1336;
dc.description.abstractThe development of simple, reliable, and rapid approaches for molecular detection of common mutations is important for prevention and early diagnosis of genetic diseases, including Thalessemia. Oligonucleotide-gated mesoporous nanoparticles-based analysis is a new platform for mutation detection that has the advantages of sensitivity, rapidity, accuracy, and convenience. A specific mutation in beta-thalassemia, one of the most prevalent inherited diseases in several countries, was used as model disease in this study. An assay for detection of IVS110 point mutation (A > G reversion) was developed by designing probe-gated mesoporous silica nanoparticles (MCM-41) loaded with reporter fluorescein molecules. The silica nanoparticles were characterized by AFM, TEM and BET analysis for having 180 nm diameter and 2.83 nm pore size regular hexagonal shape. Amine group functionalized nanoparticles were analysed with FIR technique. Mutated and normal sequence probe oligonucleotides)about 12.7 nmol per mg nanoparticles) were used to entrap reporter fluorescein molecules inside the pores and hybridization with single stranded DNA targets amplified by PCR gave different fluorescent signals for mutated targets. Samples from IVS110 mutated and normal patients resulted in statistically significant differences when the assay procedure were applied. (C) 2017 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.ab.2017.09.004
dc.identifier.endpage83en_US
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.pmid28893561
dc.identifier.scopus2-s2.0-85029282760
dc.identifier.scopusqualityQ2
dc.identifier.startpage78en_US
dc.identifier.urihttps://doi.org/10.1016/j.ab.2017.09.004
dc.identifier.urihttps://hdl.handle.net/20.500.12939/922
dc.identifier.volume537en_US
dc.identifier.wosWOS:000417116700015
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorErcan, Meltem
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofAnalytical Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSingle Nucleotide Polymorphismen_US
dc.subjectThalassemiaen_US
dc.subjectBiosensorsen_US
dc.subjectSilica Nanoparticlesen_US
dc.subjectDNA Gatesen_US
dc.titleGenotyping of single nucleotide polymorphism by probe-gated silica nanoparticles
dc.typeArticle

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