Structural effects of simvastatin on liver rate tissue: Fourier transform infrared and Raman microspectroscopic studies

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dc.contributor.authorGarip, Şebnem
dc.contributor.authorBayari, Sevgi Haman
dc.contributor.authorSevercan, Mete
dc.contributor.authorAbbas, Sherif
dc.contributor.authorLednev, Igor K.
dc.contributor.authorSevercan, Feride
dc.date.accessioned2021-05-15T12:37:17Z
dc.date.available2021-05-15T12:37:17Z
dc.date.issued2016
dc.departmentTıp Fakültesien_US
dc.descriptionAbbas, Sherif/0000-0001-8631-5027; Lednev, Igor K/0000-0002-6504-531X
dc.description.abstractSimvastatin is one of the most frequently prescribed statins because of its efficacy in the treatment of hypercholesterolemia, reducing cardiovascular risk and related mortality. Determination of its side effects on different tissues is mandatory to improve safe use of this drug. In the present study, the effects of simvastatin on molecular composition and structure of healthy rat livers were investigated by Fourier transform infrared and Raman imaging. Simvastatin-treated groups received 50 mg/kg/day simvastatin for 30 days. The ratio of the area and/or intensity of the bands assigned to lipids, proteins, and nucleic acids were calculated to get information about the drug-induced changes in tissues. Loss of unsaturation, accumulation of end products of lipid peroxidation, and alterations in lipid-to-protein ratio were observed in the treated group. Protein secondary structure studies revealed significant decrease in alpha-helix and increase in random coil, while native beta-sheet decreases and aggregated beta-sheet increases in treated group implying simvastatin-induced protein denaturation. Moreover, groups were successfully discriminated using principal component analysis. Consequently, high-dose simvastatin treatment induces hepatic lipid peroxidation and changes in molecular content and protein secondary structure, implying the risk of liver disorders in drug therapy. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)en_US
dc.description.sponsorshipHigher Education Council of TurkeyMinistry of National Education - Turkeyen_US
dc.description.sponsorshipS. Bayari would like to thank the Higher Education Council of Turkey for the fellowship to visit Igor Lednev lab to perform Raman imaging studies. We thank Dr. Alexander Rzhevskii and Thermo Fisher Scientific Inc. for their advisement and expertise and access to their DXR Raman microscope.en_US
dc.identifier.doi10.1117/1.JBO.21.2.025008
dc.identifier.issn1083-3668
dc.identifier.issn1560-2281
dc.identifier.issue2en_US
dc.identifier.pmid26891599
dc.identifier.scopus2-s2.0-84959196720
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1117/1.JBO.21.2.025008
dc.identifier.urihttps://hdl.handle.net/20.500.12939/508
dc.identifier.volume21en_US
dc.identifier.wosWOS:000371735000011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGarip, Şebnem
dc.language.isoen
dc.publisherSpie-Soc Photo-Optical Instrumentation Engineersen_US
dc.relation.ispartofJournal of Biomedical Optics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSimvastatinen_US
dc.subjectLiveren_US
dc.subjectFourier Transform Infrared Imagingen_US
dc.subjectRaman Imagingen_US
dc.subjectProtein Secondary Structureen_US
dc.subjectPrincipal Component Analysisen_US
dc.titleStructural effects of simvastatin on liver rate tissue: Fourier transform infrared and Raman microspectroscopic studies
dc.typeArticle

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