Results of real-time multiplex polymerase chain reaction assay in renal transplant recipients with sterile pyuria

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dc.contributor.authorSarıer, M.
dc.contributor.authorDemir, M.
dc.contributor.authorGöktaş, S.
dc.contributor.authorDuman, I.
dc.contributor.authorBüyükkınacı, M.
dc.contributor.authorYüksel, Y.
dc.contributor.authorŞengül, A.
dc.contributor.authorYavuz, A. H.
dc.date.accessioned2021-05-15T12:43:04Z
dc.date.available2021-05-15T12:43:04Z
dc.date.issued2017
dc.departmentTıp Fakültesien_US
dc.descriptionSarier, Mehmet/0000-0002-8656-7416; Goktas, Serdar/0000-0001-6538-7187
dc.description.abstractUrinary tract infections are a major cause of morbidity and hospitalization after renal transplantation. Patients treated with immunosuppressive drugs suffer not only from common uropathogens but also from opportunistic infections caused by unusual uropathogens. Sterile pyuria is associated with numerous infectious agents including viruses, fungi, and atypical or fastidious organisms. The objective of this study was to investigate the pathogens using real-time multiplex polymerase chain reaction (rtMPCR) assay in sterile pyuria of renal transplant recipients. In this prospective controlled study, pathogen detection was performed with rtMPCR assay on October 2016 in 60 patients with sterile pyuria who had undergone kidney transplantation. A total of 40 renal transplant patients were determined as the control group. Male-to-female ratio was same. The mean age of the subjects with sterile pyuria was 45.7 +/- 12.1 (25-74). The mean duration after transplantation was 28.8 +/- 3.97 (3-102) months. Pathogens were detected with rtMPCR in 61.7% of sterile pyuria group. This rate was significantly higher compared with the control group (P < .001). Two or more different pathogens were found in 13 (21.7%) patients in sterile pyuria group. The pathogens found included cytomegalovirus in 10 patients (19%), Gardnerella vaginalis and obligate anaerobes in 20 patients (38%), Ureaplasma spp in 17 patients (33%), Candida spp in 2 patients (4%), Mycoplasma hominis in one patient (2%), herpes simplex virus-2 in one patient (2%), and Trichomonas vaginalis in one patient (2%). Sterile pyuria may indicate the presence of genitourinary pathogens that cannot be detected with conventional urine culture method in renal transplantation patients. rtMPCR is an accurate and convenient method for detection of multiple potential pathogens of sterile pyuria in renal transplant patients.en_US
dc.identifier.doi10.1016/j.transproceed.2017.02.051
dc.identifier.endpage1311en_US
dc.identifier.issn0041-1345
dc.identifier.issn1873-2623
dc.identifier.issue6en_US
dc.identifier.pmid28735999
dc.identifier.scopus2-s2.0-85024888697
dc.identifier.scopusqualityQ3
dc.identifier.startpage1307en_US
dc.identifier.urihttps://doi.org/10.1016/j.transproceed.2017.02.051
dc.identifier.urihttps://hdl.handle.net/20.500.12939/1004
dc.identifier.volume49en_US
dc.identifier.wosWOS:000406734700018
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDemir, M.
dc.language.isoen
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTransplantation Proceedings
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMultiplex Polymeraseen_US
dc.subjectMedicineen_US
dc.subjectRenal Transplanten_US
dc.titleResults of real-time multiplex polymerase chain reaction assay in renal transplant recipients with sterile pyuria
dc.typeArticle

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