Improvement stability and performance of invertase via immobilization on to silanized and polymer brush grafted magnetic nanoparticles

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorDoz, Tuğce
dc.contributor.authorÖzalp, Veli Cengiz
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2021-05-15T12:36:39Z
dc.date.available2021-05-15T12:36:39Z
dc.date.issued2017
dc.departmentTıp Fakültesien_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990;
dc.description.abstractIn this study, magnetic nanoparticles (Fe3O4) were modified sequentially with silica (Fe3O4@SiO2), glycidyl methacrylate (GMA) by surface initiated atom transfer radical polymerization (SI-ATRP) and hexamethylene diamine (as a spacer arm). The p(GMA) grafted and SA modified form (i.e., Fe3O4@SiO2@pGMA-SA-3) was used for covalent immobilization of invertase (EC 3.2.1.26). The amount of immobilized enzyme on Fe3O4@SiO2@p(GMA) and Fe3O4@SiO2@p(GMA)-SA-3 was 36.1 +/- 0.9 and 33.4 +/- 1.3 mg/g, respectively. The K-m and V-max values of immobilized invertase were found to be 39.4 mmol/L and 349.5 mmol/L min, and not significantly changed compared with free form (34.3 mmol/L and 387.2 mmol/L min), respectively, revealed that the applied protocol did not have any detrimental effect on the retained activity of immobilized invertase. (C) 2016 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.foodchem.2016.11.007
dc.identifier.endpage1450en_US
dc.identifier.issn0308-8146
dc.identifier.issn1873-7072
dc.identifier.pmid27979113
dc.identifier.scopus2-s2.0-85006324355
dc.identifier.scopusqualityQ1
dc.identifier.startpage1442en_US
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2016.11.007
dc.identifier.urihttps://hdl.handle.net/20.500.12939/364
dc.identifier.volume221en_US
dc.identifier.wosWOS:000389909100184
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBayramoğlu, Gülay
dc.institutionauthorÖzalp, Veli Cengiz
dc.institutionauthorArıca, M. Yakup
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMagnetic Supporten_US
dc.subjectImmobilizationen_US
dc.subjectInvertaseen_US
dc.subjectSucrose Hydrolysisen_US
dc.subjectKinetic Parameteren_US
dc.subjectContinuous Systemen_US
dc.titleImprovement stability and performance of invertase via immobilization on to silanized and polymer brush grafted magnetic nanoparticles
dc.typeArticle

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