Fibrous polymer grafted magnetic chitosan beads with strong poly(cation-exchange) groups for single step purification of lysozyme

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorTekinay, Turgay
dc.contributor.authorÖzalp, Veli Cengiz
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2021-05-15T12:37:51Z
dc.date.available2021-05-15T12:37:51Z
dc.date.issued2015
dc.departmentTıp Fakültesi, Biyokimya Anabilim Dalıen_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990
dc.description.abstractLysozyme is an important polypetide used in medical and food applications. We report a novel magnetic strong cation exchange beads for efficient purification of lysozyme from chicken egg white. Magnetic chitosan (MCHT) beads were synthesized via phase inversion method, and then grafted with poly(glycidyl methacrylate) (p(GMA)) via the surface-initiated atom transfer radical polymerization (SI-ATRP). Epoxy groups of the grafted polymer, were modified into strong cation-exchange groups (i.e., sulfonate groups) in the presence of sodium sulfite. The MCTH and MCTH-g-p(GMA)-SO3H beads were characterized by ATR-FTIR, SEM, and VSM. The sulphonate groups content of the modified MCTH-g-p(GMA)-4 beads was found to be 0.53 mmol g(-1) of beads by the potentiometric titration method. The MCTH-g-p(GMA)-SO3H beads were first used as an ion-exchange support for adsorption of lysozyme from aqueous solution. The influence of different experimental parameters such as pH, contact time, and temperature on the adsorption process was evaluated. The maximum adsorption capacity was found to be 208.7 mg g(-1) beads. Adsorption of lysozyme on the MCTH-g-p(GMA)-SO3H beads fitted to Langmuir isotherm model and followed the pseudo second-order kinetic. More than 93% of the adsorbed lysozyme was desorbed using Na2CO3 solution (pH 11.0). The purity of the lysozyme was checked by HPLC and SDS gel electrophoresis. In addition, the MCTH-g-p(GMA)-SO3H beads prepared in this work showed promising potential for separation of various anionic molecules. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.jchromb.2015.03.030
dc.identifier.endpage95en_US
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.pmid25864009
dc.identifier.scopus2-s2.0-84926390543
dc.identifier.scopusqualityQ2
dc.identifier.startpage84en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2015.03.030
dc.identifier.urihttps://hdl.handle.net/20.500.12939/595
dc.identifier.volume990en_US
dc.identifier.wosWOS:000354155000012
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzalp, Veli Cengiz
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChitosanen_US
dc.subjectMagnetic Beadsen_US
dc.subjectIon-Exchange Beadsen_US
dc.subjectAdsorptionen_US
dc.subjectPurificationen_US
dc.subjectLysozymeen_US
dc.titleFibrous polymer grafted magnetic chitosan beads with strong poly(cation-exchange) groups for single step purification of lysozyme
dc.typeArticle

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