Investigation of the effects of various stress factors on biofilms and planktonic bacteria in cooling tower model system

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dc.contributor.authorVatansever, Cansu
dc.contributor.authorTüretgen, İrfan
dc.date.accessioned2021-05-15T11:33:18Z
dc.date.available2021-05-15T11:33:18Z
dc.date.issued2021
dc.departmentDiş Hekimliği Fakültesien_US
dc.descriptionTuretgen, Irfan/0000-0002-7866-1007; Turetgen, Irfan/0000-0002-7866-1007
dc.description.abstractBiofilm is a microbial population which live in a self-produced extracellular polymeric matrix by attaching to surfaces. Biofilms consist of different different types of organisms such as bacteria, fungi, protozoa, etc. Many biofilms that develop in nature consist of more than one type of organism. Biofilms protect bacteria from adverse conditions such as temperature fluctuation and disinfectants. The aim of this study was to determine the effective elimination strategies for combating biofilm and planktonic bacteria in cooling tower model system using different decontamination / disinfection techniques. In this study, 14 week-old biofilms were treated with temperatures of 4 degrees C, 65 degrees C; pH of 3, 11; 2 and 10 mg/l chlorine, 2 and 10 mg/l monochloramine; hypotonic salt (0.01% NaCl) and hypertonic salt (3% NaCl) solution. For enumeration, number of aerobic heterotrophic bacteria was determined by conventional culture method, number of live bacteria was determined by LIVE/DEAD viability kit, CTC-DAPI and Alamar blue staining methods. Temperature of 65 degrees C, pH of 3, 10 mg/l monochloramine and hypertonic salt solution were the most effective parameters for decontamination of biofilm and planktonic bacteria. Biofilm bacteria in the circulating water system were significantly more resistant than planktonic bacteria against stress factors. When the numbers of epifluorescence microscopy and conventional culture technique were compared, significantly higher number of live bacteria were detected using epifluorescence microscopy. Bacteria enter the viable but non-culturable phase by loosing their culturability under stress conditions. For this reason, the conventional culture method should be supported by different techniques to get more realistic numbers.en_US
dc.description.sponsorshipIstanbul UniversityIstanbul University [38263]en_US
dc.description.sponsorshipThis work was funded by 'Research Fund of the Istanbul University' (Project Code: 38263).en_US
dc.identifier.doi10.1007/s00203-020-02116-2
dc.identifier.endpage1425en_US
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue4en_US
dc.identifier.pmid33388788
dc.identifier.scopus2-s2.0-85098652502
dc.identifier.scopusqualityQ3
dc.identifier.startpage1411en_US
dc.identifier.urihttps://doi.org/10.1007/s00203-020-02116-2
dc.identifier.urihttps://hdl.handle.net/20.500.12939/123
dc.identifier.volume203en_US
dc.identifier.wosWOS:000604476800002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorVatansever, Cansu
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiofilmen_US
dc.subjectStress Factorsen_US
dc.subjectCooling Toweren_US
dc.subjectEpifluorescence Microscopyen_US
dc.subjectLIVEen_US
dc.subjectDEAD Kiten_US
dc.titleInvestigation of the effects of various stress factors on biofilms and planktonic bacteria in cooling tower model system
dc.typeArticle

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