Nanoparticle embedded enzymes for improved lateral flow sensors

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dc.contributor.authorÖzalp, Veli Cengiz
dc.contributor.authorZeydanlı, Uğur S.
dc.contributor.authorLunding, Anita
dc.contributor.authorKavruk, Murat
dc.contributor.authorÖz, M. Tufan
dc.contributor.authorEyidoğan, Füsun
dc.contributor.authorÖktem, Huseyin A.
dc.date.accessioned2021-05-15T12:41:03Z
dc.date.available2021-05-15T12:41:03Z
dc.date.issued2013
dc.departmentEczacılık Fakültesien_US
dc.descriptionOz, Mehmet Tufan/0000-0002-0042-2671; Kavruk, Murat/0000-0001-5331-7253; Ozalp, Veli Cengiz/0000-0002-7659-5990; Olsen, Lars Folke/0000-0001-7391-2340
dc.description.abstractIn this study, combining the nanoparticle embedded sensors with lateral flow assays, a novel strategy for ensuring the quality of signalling in lateral flow assays (LFAs) was developed. A LFA for reactive oxygen species (ROS) is reported that is based on horse radish peroxidase (HRP) which is co-entrapped with Texas Red dextran inside porous polyacrylamide nanoparticles. In this system, enzymes are protected in the porous matrix of polyacrylamide which freely allows the diffusion of the analyte. The sensor is rapid and sensitive for quantification of hydrogen peroxide concentrations. A test solution of hydrogen peroxides was quantified with this novel LFA-ROS sensor to obtain a linear range between 1 and 25 mM. Nanoparticle embedding of enzymes is proposed here as a general strategy for developing enzyme-based lateral flow assays, eliminating adverse effects associated with biological samples.en_US
dc.identifier.doi10.1039/c3an00733b
dc.identifier.endpage4259en_US
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.issue15en_US
dc.identifier.pmid23730687
dc.identifier.scopus2-s2.0-84879762297
dc.identifier.scopusqualityQ1
dc.identifier.startpage4255en_US
dc.identifier.urihttps://doi.org/10.1039/c3an00733b
dc.identifier.urihttps://hdl.handle.net/20.500.12939/736
dc.identifier.volume138en_US
dc.identifier.wosWOS:000321152300003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzalp, Veli Cengiz
dc.language.isoen
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofAnalyst
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDAB-Peroxidase Activity Assayen_US
dc.titleNanoparticle embedded enzymes for improved lateral flow sensors
dc.typeArticle

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