Discrimination of heavy metal acclimated environmental strains by chemometric analysis of FTIR spectra

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dc.contributor.authorKepenek, Eda Şeyma
dc.contributor.authorSevercan, Mete
dc.contributor.authorGözen, Ayşe Gül
dc.contributor.authorSevercan, Feride
dc.date.accessioned2021-05-15T11:33:29Z
dc.date.available2021-05-15T11:33:29Z
dc.date.issued2020
dc.departmentTıp Fakültesi, Biyofizik Anabilim Dalıen_US
dc.description.abstractHeavy metal acclimated bacteria are profoundly the preferred choice for bioremediation studies. Bacteria get acclimated to toxic concentrations of heavy metals by induction of specific enzymes and genetic selection favoring new metabolic abilities leading to activation of one or several of resistance mechanisms creating bacterial populations with differences in resistance profile and/or level. Therefore, to use in bioremediation processes, it is important to discriminate acclimated bacterial populations and choose a more resistant strain. In this study, we discriminated heavy metal acclimated bacteria by using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy and multivariate analysis methods namely Hierarchical Cluster Analysis (HCA), Principal Component Analysis (PCA) and Soft Independent Modeling of Class Analogy (SIMCA). Two acclimation methods, acute and gradual, were used which cause differences in molecular changes resulting in bacterial populations with different molecular and resistance profiles. Brevundimonas sp., Gordonia sp., and Microbacterium oxydans were exposed to the toxic concentrations of Cd (30 mu g/ml) or Pb (90 mu g/ml) by using broth medium as a growth media. Our results revealed that PCA and HCA clearly discriminated the acute-acclimated, gradual acclimated, and control bacteria from each other in protein, carbohydrate, and whole spectral regions. Furthermore, we classified acclimated (acute and gradual) and control bacteria more accurately by using SIMCA with 99.9% confidence. This study demonstrated that heavy metal acclimated and control group bacteria can be discriminated by using chemometric analysis of FTIR spectra in a powerful, cost-effective, and handy way. In addition to the determination of the most appropriate acclimation procedure, this approach can be used in the detection of the most resistant bacterial strains to be used in bioremediation studies.en_US
dc.description.sponsorshipEnvironment, Atmosphere, Earth and Marine Sciences Research Group (CAYDAG) of the Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Y515]en_US
dc.description.sponsorshipThis work was supported by the Environment, Atmosphere, Earth and Marine Sciences Research Group (CAYDAG) of the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No. 113Y515).en_US
dc.identifier.doi10.1016/j.ecoenv.2020.110953
dc.identifier.issn0147-6513
dc.identifier.issn1090-2414
dc.identifier.pmid32800227
dc.identifier.scopus2-s2.0-85087742099
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.ecoenv.2020.110953
dc.identifier.urihttps://hdl.handle.net/20.500.12939/171
dc.identifier.volume202en_US
dc.identifier.wosWOS:000564336200005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSevercan, Feride
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofEcotoxicology and Environmental Safety
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacteriaen_US
dc.subjectATR-FTIRen_US
dc.subjectPCAen_US
dc.subjectHCAen_US
dc.subjectSIMCAen_US
dc.subjectPben_US
dc.subjectCden_US
dc.titleDiscrimination of heavy metal acclimated environmental strains by chemometric analysis of FTIR spectra
dc.typeArticle

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