Structural effects of simvastatin on rat liver tissue: Fourier transform infrared and Raman microspectroscopic studies (vol 21, 025008, 2016)
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Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Spie-Soc Photo-Optical Instrumentation Engineers
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Simvastatin is one of the most frequently prescribed statins because of its efficacy in the treatment of hypercholesterolemia, reducing cardiovascular risk and related mortality. Determination of its side effects on different tissues is mandatory to improve safe use of this drug. In the present study, the effects of simvastatin on molecular composition and structure of healthy rat livers were investigated by Fourier transform infrared and Raman imaging. Simvastatin-treated groups received 50 mg/kg/day simvastatin for 30 days. The ratio of the area and/or intensity of the bands assigned to lipids, proteins, and nucleic acids were calculated to get information about the drug-induced changes in tissues. Loss of unsaturation, accumulation of end products of lipid peroxidation, and alterations in lipid-to-protein ratio were observed in the treated group. Protein secondary structure studies revealed significant decrease in alpha-helix and increase in random coil, while native beta-sheet decreases and aggregated beta-sheet increases in treated group implying simvastatin-induced protein denaturation. Moreover, groups were successfully discriminated using principal component analysis. Consequently, high-dose simvastatin treatment induces hepatic lipid peroxidation and changes in molecular content and protein secondary structure, implying the risk of liver disorders in drug therapy. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Açıklama
Abbas, Sherif/0000-0001-8631-5027; Lednev, Igor K/0000-0002-6504-531X
Anahtar Kelimeler
Simvastatin, Liver, Fourier Transform Infrared Imaging, Raman Imaging, Protein Secondary Structure, Principal Component Analysis
Kaynak
Journal of Biomedical Optics
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
21
Sayı
4
