Comparative Proteomic Analysis of Dental-Origin Stem Cells: Insights into Regenerative Potential

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dc.contributor.authorTez, Banu Çiçek
dc.contributor.authorDurukan, Sebahat Melike
dc.contributor.authorYıldır, Selin Kübra
dc.contributor.authorÇokkeçeci, Murat
dc.contributor.authorBoyvat, Dudu
dc.contributor.authorAltınsoy, Nilay
dc.contributor.authorFındık, Fatma
dc.contributor.authorAyaz Güner, Şerife
dc.contributor.authorAcar, Mustafa Burak
dc.contributor.authorGelderisi, Umberto
dc.contributor.authorÖzcan, Servet
dc.date.accessioned2025-08-14T13:59:45Z
dc.date.available2025-08-14T13:59:45Z
dc.date.issued2025
dc.departmentFakülteler, Diş Hekimliği Fakültesi, Restoratif Diş Tedavisi Ana Bilim Dalı
dc.description.abstractTeeth are a significant source of stem cells and have clinical importance for regenerative medicine. A human tooth harbors different kinds of stem cells in the dental pulp (DPSC) or the periodontal ligament (PDLSC). Also exfoliated teeth in childhood contain a special type of stem cells in their pulp called Stem cells from Human Exfoliated Deciduous teeth (SHED). All these stem cells have features and capacities that vary depending on their niche. Here we investigated the proteomic properties of three types of stem cells that originated from human teeth. We isolated and cultured the DPSCs, PDLSCs, and SHED cells. After validating MSC populations via immunophenotyping, we performed a mass spectrometry-based proteomic approach to identify and relatively quantify whole cell and secreted proteins. Identified proteins were evaluated by using Gene Ontology and Reactome pathway analysis tools. Our data reveal that SHED cells represented inflammation, hypoxia, and nutrient deficiency-associated ontologies in both their secretome and whole-cell proteomes. The whole-cell proteome of PDLSCs consisted of differentiation and proliferation-associated molecules while their secretory molecules were mainly associated with inflammation, ECM organization, and immune response. Among dental-originated stem cells, DPSCs appeared to be the healthiest and clinically relevant in terms of proteomic properties with their proliferation, growth factor signaling, and stemness-associated molecules in their secretome and whole-cell proteome. Obtained results demonstrated that every type of stem cell from dental origin has unique proteomic features that are altered by their location and physiological conditions. The findings may help researchers improve the dental stem-cell-based regenerative medicine approaches.
dc.identifier.citationTez, B. Ç., Durukan, S. M., Yıldır, S. K., Çokkeçeci, M., Boyvat, D., Altınsoy, N., ... & Özcan, S. (2025). Comparative Proteomic Analysis of Dental-Origin Stem Cells: Insights into Regenerative Potential. Stem Cell Reviews and Reports, 1-11. 10.1007/s12015-025-10936-2
dc.identifier.doi10.1007/s12015-025-10936-2
dc.identifier.issn2629-3277
dc.identifier.issn2629-3269
dc.identifier.pmid40685492
dc.identifier.scopus2-s2.0-105011190470
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://hdl.handle.net/20.500.12939/5844
dc.identifier.wosWOS:001531435600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakPubMed
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorDurukan, Sebahat Melike
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofStem Cell Reviews and Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDPSC
dc.subjectPDLSC
dc.subjectProteome
dc.subjectSHED
dc.subjectSecretome
dc.titleComparative Proteomic Analysis of Dental-Origin Stem Cells: Insights into Regenerative Potential
dc.typeArticle

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