Gene expression profile as a precursor of inflammation in mouse models: BFMI860 and C57BL/6NCrl

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dc.contributor.authorDoğan, Ayça
dc.contributor.authorBrockmann, Gudrun A.
dc.date.accessioned2024-09-17T07:03:18Z
dc.date.available2024-09-17T07:03:18Z
dc.date.issued2024en_US
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri, Fizyoloji Ana Bilim Dalıen_US
dc.description.abstractObjective: We aimed to investigate the differences in the immune response to body fat content between the genetically mutant obese BFMI860 (BFMI) mouse strain and the lean C57BL/6Ncrl (B6) mouse strain as a control and the effects of obesity on gene expression on inflammation-related pathways in epididymal adipose tissue. Materials and Methods: Six males from each strain were maintained on a standard maintenance diet (SMD) or a high-fat diet (HFD). At the age of 10 weeks, serum and epididymal adipose tissue samples were collected for cytokine and gene expression analyses. RNA samples from epididymal adipose tissue were hybridized using the microarray technique to study the quantitative transcript amounts of genes. Results: Pathway analysis of gene expression data revealed no considerable development of inflammatory state in BFMI and B6 on SMD. Both strains responded to HFD distinctly; the inflammatory state was more prominent in the obese BFMI group than in the lean B6 group. Several genes, such as Adipoq, NFkbia, Plaur, F2r, C3ar1, and Nfatc4 in pathways involved in the immune system have been found to be differentially regulated in BFMI mice. Under the condition of obesity in BFMI mice, the induction of inflammation-related pathways indicates an increased risk of insulin resistance, atherosclerosis, and cardiovascular disease. Conclusion: This study identified distinct expression patterns of genes involved in inflammatory pathways, particularly those associated with the adipocytokine signaling pathway and complement and coagulation cascades, in the epididymal adipose tissue of BFMI and B6 mice. The BFMI strain is a valuable and promising model for clarifying the mechanisms underlying obesity and the activation of inflammation in adipose tissue.en_US
dc.identifier.citationDoğan, A., Brockmann, G. A. (2024). Gene expression profile as a precursor of inflammation in mouse models: BFMI860 and C57BL/6NCrl. Experimed, 14(2), 73-84. 10.26650/experimed.1384602en_US
dc.identifier.endpage84en_US
dc.identifier.issn2667-5846
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85202769778
dc.identifier.scopusqualityQ4
dc.identifier.startpage73en_US
dc.identifier.trdizinid1277160
dc.identifier.urihttps://hdl.handle.net/20.500.12939/4917
dc.identifier.volume14en_US
dc.identifier.wosWOS:001322096300003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorDoğan, Ayça
dc.language.isoen
dc.publisherIstanbul University Pressen_US
dc.relation.ispartofExperimed
dc.relation.isversionof10.26650/experimed.1384602en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdipocytokine signaling pathwayen_US
dc.subjectComplement and coagulation cascadeen_US
dc.subjectHigh-fat dieten_US
dc.subjectObesityen_US
dc.titleGene expression profile as a precursor of inflammation in mouse models: BFMI860 and C57BL/6NCrl
dc.typeArticle

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