Modified chitosan-based nanoadjuvants enhance immunogenicity of protein antigens after mucosal vaccination
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Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Nasal vaccination is considered to be an effective and convenient way of increasing immune responses both systemically and locally. Although various nanovaccine carriers have been introduced as potential immune adjuvants, further improvements are still needed before they can be taken to clinical usage. Chitosan-based nanovaccine carriers are one of the most widely studied adjuvants, owing to the ability of chitosan to open tight junctions between nasal epithelial cells and enhance particle uptake as well as its inherent immune activating role. In present study, bovine serum albumin (BSA) loaded nanoparticles were prepared using novel aminated (aChi) and aminated plus thiolated chitosan (atChi) polymers, to further enhance mucoadhesiveness and adjuvanticity of the vaccine system by improving electrostatic interactions of polymers with negatively charged glycoproteins. Nanocarriers with optimum size and surface charge, high encapsulation efficiency of model antigen and good stability were developed. Negligible toxicity was observed in Calu-3 and A549 cell lines. In vivo studies, revealed high levels of systemic antibodies (IgG, IgG(1) and IgG(2a)) throughout the study and presence of sIgA in vaginal washes showed that common mucosal system was successfully stimulated. Cytokine levels indicated a mixed Th1/Th2 immune response. A shift towards cellular immune responses was observed after nasal immunisation with antigen loaded nanoparticle formulations. These nanoparticles exhibit great potential for nasal application of vaccines.
Açıklama
OZGUMUS, SAADET/0000-0002-1793-0859; Cevher, Erdal/0000-0002-0486-2252; GOK, MEHMET KORAY/0000-0003-2497-9359
Anahtar Kelimeler
Nanoparticles, Nasal Delivery, Mucosal Vaccination, Chitosan, Antibody, Cytokine
Kaynak
International Journal of Pharmaceutics
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
569
