Prognostic evidence of LEF1 isoforms in childhood acute lymphoblastic leukemia

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dc.contributor.authorErbilgin, Yücel
dc.contributor.authorNg, Özden Hatırnaz
dc.contributor.authorCan, İsmail
dc.contributor.authorFırtına, Sinem
dc.contributor.authorKüçükcankurt, Fulya
dc.contributor.authorKaraman, Serap
dc.contributor.authorSayıtoğlu, Müge
dc.contributor.authorGelen, Sema Aylan
dc.contributor.authorÖzdemir, Gül Nihal
dc.contributor.authorYıldırmak, Yıldız
dc.contributor.authorDoğru, Ömer
dc.contributor.authorTansel, Türkan
dc.contributor.authorKhodzhaev, Khusan
dc.contributor.authorToluk, Özlem
dc.contributor.authorÖzbek, Uğur
dc.date.accessioned2021-05-15T11:16:19Z
dc.date.available2021-05-15T11:16:19Z
dc.date.issued2021
dc.departmentTıp Fakültesien_US
dc.descriptionDOGRU, Omer/0000-0002-2528-2409; FIRTINA, Sinem/0000-0002-3370-8545; Hatirnaz Ng, Ozden/0000-0001-7728-6527
dc.description.abstractIntroduction The lymphoid enhancer factor 1 (LEF1) is a DNA-binding transcription factor that functions in the Wnt signaling pathway. Increased LEF1 activity is associated with progression of several types of cancer including leukemia. Here, we investigated LEF1 isoform expression and genomic variations in acute lymphoblastic leukemia (ALL). Methods LEF1 isoform expression was evaluated by quantitative real-time PCR in 87 newly diagnosed childhood ALL patients and controls. Moreover, Western blot analysis was performed for detection of LEF1 expression and the hotspot region of LEF1 was screened by deep sequencing. Results The LEF1 mRNA expression of B cell ALL patients was higher than the controls (LEF1-total P = .011, LEF1-long P = .026). Moreover, B-ALL samples showing higher total LEF1 expression had significantly shorter relapse-free survival (P = .008) and overall survival (P = .011). Although full-length LEF1 expression was similar to the controls in T-ALL, 50% (n = 15) of the ALL patients had increased full-length LEF1 protein expression. Imbalance between short- and full-length LEF1 isoforms may lead to cell survival in ALL. Beside the LEF1 activation, LEF1 gene variations were rarely observed in our cohort. Conclusion The results indicate that the Wnt pathway may have a pathogenic function in a group of ALL patients and high LEF1-total expression might be a marker for shorter relapse-free survival time in B cell ALL.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul UniversityIstanbul University [22740]; Turkish Society of Hematology Research Funden_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul University, Grant/Award Number: 22740; Turkish Society of Hematology Research Funden_US
dc.identifier.doi10.1111/ijlh.13513
dc.identifier.issn1751-5521
dc.identifier.issn1751-553X
dc.identifier.pmid33844466
dc.identifier.scopus2-s2.0-85104151982
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1111/ijlh.13513
dc.identifier.urihttps://hdl.handle.net/20.500.12939/94
dc.identifier.wosWOS:000639287900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKüçükcankurt, Fulya
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofInternational Journal of Laboratory Hematology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectALLen_US
dc.subjectBiomarkeren_US
dc.subjectGene Expressionen_US
dc.subjectLEF1en_US
dc.subjectPrognosisen_US
dc.titlePrognostic evidence of LEF1 isoforms in childhood acute lymphoblastic leukemia
dc.typeArticle

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