The inhibitory effects of amylase and streptokinase on minimum inhibitory concentration of antibiotics used to treat gram negative bacteria biofilm infection on indwelling devices

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dc.contributor.authorAksoy, Nilay
dc.contributor.authorVatansever, Cansu
dc.contributor.authorAdalı, Ceren
dc.contributor.authorAdaklı Aksoy, Başak
dc.contributor.authorFışgın, Tunç
dc.date.accessioned2023-11-24T08:23:47Z
dc.date.available2023-11-24T08:23:47Z
dc.date.issued2023en_US
dc.departmentFakülteler, Eczacılık Meslek Bilimleri Bölümü, Klinik Eczacılık Ana Bilim Dalıen_US
dc.description.abstractThe study evaluated and compared the effect of adding streptokinase and amylase to antibiotics that are already used in clinical practice to treat Gram negative bacteria biofilm infection on indwelling devices on the antibiotics' minimum inhibitory concentration (MIC). 24 h-old biofilms were developed on 96-well plate with eight clinical isolates. MIC of amikacin, cefepime, ceftazidime, colistin, meropenem, and piperacillin–tazobactam, on biofilms were measured before and after the addition of 25 U/ml streptokinase and 25 μg/ml amylase with microplate reader. The addition of streptokinase reduces the MICs of cefepime, ceftazidime, colistin, meropenem from (16, 16, 8, 4 μg/ml) to (8, 1, 1, 0.5 μg/ml) in Escherichia coli (isolate 1). While the addition of amylase reduces the MICs of only cefepime, ceftazidime from (16, 16 μg/ml) to (2, 4 μg/ml) in E. coli (isolate 1). In Pseudomonas aeruginosa (isolate 4), the MICs of amikacin, cefepime, ceftazidime, colistin and meropenem (64, 16, 32, 4, 32 μg/ml) reduced to (2, 1, 0.5, 0.25, 0.5 μg/ml) with streptokinase and (4, 4, 4, 2, 0.5 μg/ml) with amylase respectively. Similar inhibitions were seen in Pseudomonas putida, Proteus mirabilis. We can conclude that the addition of streptokinase and amylase were effective in reducing the MICs of antibiotics that are commonly used to treat Gram negative bacteria biofilm infection on indwelling devices, thereby increasing susceptibility of bacteria to antibiotics. Streptokinase obviously had a greater effect than amylase, implying that it should be prioritized in future in vivo and clinical studies to obtain successful therapy with antibiotics on biofilm infections.en_US
dc.identifier.citationAksoy, N., Vatansever, C., Adalı, C., Adaklı Aksoy, B., Fışgın, T. (2023). The inhibitory effects of amylase and streptokinase on minimum inhibitory concentration of antibiotics used to treat gram negative bacteria biofilm infection on indwelling devices. Indian Journal of Microbiology, 63(4), 533-540.en_US
dc.identifier.endpage540en_US
dc.identifier.issn0046-8991
dc.identifier.issn0973-7715
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85174909405
dc.identifier.scopusqualityQ2
dc.identifier.startpage533en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12939/4239
dc.identifier.volume63en_US
dc.identifier.wosWOS:001092070700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAksoy, Nilay
dc.institutionauthorVatansever, Cansu
dc.institutionauthorAdaklı Aksoy, Başak
dc.institutionauthorFışgın, Tunç
dc.language.isoen
dc.relation.ispartofIndian Journal of Microbiology
dc.relation.isversionof10.1007/s12088-023-01109-1en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStreptokinaseen_US
dc.subjectAmylaseen_US
dc.subjectBioflmen_US
dc.subjectGram negative bacteriaen_US
dc.subjectInfectionsen_US
dc.titleThe inhibitory effects of amylase and streptokinase on minimum inhibitory concentration of antibiotics used to treat gram negative bacteria biofilm infection on indwelling devices
dc.typeArticle

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