Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: In vivo studies

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dc.contributor.authorCevher, Erdal
dc.contributor.authorSalomon, Stefan K.
dc.contributor.authorSomavarapu, Satyanarayana
dc.contributor.authorBrocchini, Steve
dc.contributor.authorAlpar, H. Oya
dc.date.accessioned2021-05-15T12:40:40Z
dc.date.available2021-05-15T12:40:40Z
dc.date.issued2015
dc.departmentEczacılık Fakültesien_US
dc.descriptionCevher, Erdal/0000-0002-0486-2252
dc.description.abstractHere, we aimed at developing chitosan/pullulan composite nanoparticles and testing their potential as novel systems for the nasal delivery of diphtheria toxoid (DT). All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV). Their immunological effects after intranasal administration to mice were compared to intramuscular route. Composite nanoparticles induced higher levels of IgG responses than particles formed with chitosan derivative and antigen. Nasally administered TMC-pullulan composites showed higher DT serum IgG titre when compared with the other composites. Co-encapsulation of CpG ODN within TMC-CMP-DT nanoparticles resulted in a balanced Th-1/Th-2 response. TMC/pullulan composite nanoparticles also induced highest cytokine levels compared to those of chitosan salts. These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.en_US
dc.identifier.doi10.3109/02652048.2015.1073393
dc.identifier.endpage783en_US
dc.identifier.issn0265-2048
dc.identifier.issn1464-5246
dc.identifier.issue8en_US
dc.identifier.pmid26480962
dc.identifier.scopus2-s2.0-84945495370
dc.identifier.scopusqualityQ2
dc.identifier.startpage769en_US
dc.identifier.urihttps://doi.org/10.3109/02652048.2015.1073393
dc.identifier.urihttps://hdl.handle.net/20.500.12939/646
dc.identifier.volume32en_US
dc.identifier.wosWOS:000369970100005
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAlpar, H. Oya
dc.language.isoen
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal of Microencapsulation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarboxymethyl Pullulanen_US
dc.subjectChitosan Derivativesen_US
dc.subjectIntramuscular Immunisationen_US
dc.subjectNanoparticlesen_US
dc.subjectNasal Immunisationen_US
dc.subjectPolyion Complexationen_US
dc.titleDevelopment of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: In vivo studies
dc.typeArticle

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