The impact of a-tomatine on shear bonding strength in different dentin types and on cariogenic microorganisms: an in vitro and in silico study

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dc.contributor.authorÜçüncü, Musa Kazım
dc.contributor.authorYıldırım Üçüncü, Merve
dc.contributor.authorTopçuoğlu, Nursen
dc.contributor.authorKitin, Emine
dc.contributor.authorYazıcıoğlu, Oktay
dc.contributor.authorOrtaakarsu, Ahmet Buğra
dc.contributor.authorAydın, Mustafa
dc.contributor.authorErol, Ayşe
dc.date.accessioned2024-10-23T05:53:14Z
dc.date.available2024-10-23T05:53:14Z
dc.date.issued2024en_US
dc.departmentFakülteler, Diş Hekimliği Fakültesi, Restoratif Diş Tedavisi Ana Bilim Dalıen_US
dc.description.abstractIntroduction: The objective of this study is to investigate the shear bonding strength of a glycoalkaloid, also a novel matrix metalloproteinase enzyme known as α-tomatine, on two different surfaces of dentin (sound & caries-affected) and its efficacy against cariogenic microorganisms using in vitro and in silico methods. Methods: The effect of a-tomatine at different concentrations (0.75 / 1 / 1.5 µM) on shear bonding strength in caries-affected and sound dentin was also investigated (n = 10; each per subgroup). The analysis of shear bonding and failure tests was conducted after a 24-hour storage period. Fracture surfaces were examined under a scanning electron microscope. A stock solution 3 mM of a-tomatine was prepared for antimicrobial evaluation. Antimicrobial activities of the agents against Streptococcus mutans ATCC 25175, Lactobacillus casei ATCC 4646, and Candida albicans ATCC 10231 standard strains were investigated by microdilution method. In addition, through the method of molecular docking and dynamic analysis, the affinity of a-tomatine for certain enzymes of these microorganisms was examined. Results: The pretreatment agent and dentin type significantly influenced shear bonding strength values (p < 0.05). As the molarity of a-tomatine increased, the bonding value decreased in sound dentin, while the opposite was true in caries-affected dentin. According to molecular docking and dynamic analysis, the highest affinity was observed in L. casei's signaling protein. Microdilution assays revealed a-tomatine to exhibit fungicidal activity against C. albicans and bacteriostatic effects against S. mutans. No antimicrobial effect was observed on L. casei. Conclusion: a-tomatine demonstrates a positive impact by serving as both a pretreatment agent for bonding strength and an inhibitor against certain cariogenic microorganisms.en_US
dc.identifier.citationUçuüncü, M. K., Yıldırım Üçüncü, M., Topçuoğlu, N., Kitin, E., Yazıcıoğlu, O., Ortaakarsu, A. B., Aydın, M., Erol, A. (2024). The impact of a-tomatine on shear bonding strength in different dentin types and on cariogenic microorganisms: an in vitro and in silico study. BMC Oral Health, 24(1). 10.1186/s12903-024-05001-2en_US
dc.identifier.issn1472-6831
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85206274212
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://hdl.handle.net/20.500.12939/4943
dc.identifier.volume24en_US
dc.identifier.wosWOS:001335882600007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÜçüncü, Musa Kazım
dc.language.isoen
dc.relation.ispartofBMC Oral Health
dc.relation.isversionof10.1186/s12903-024-05001-2en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCandida albicansen_US
dc.subjectCaries-affected dentinen_US
dc.subjectCariogenic microorganismsen_US
dc.subjectDental cariesen_US
dc.subjectLactobacillus caseien_US
dc.subjectStreptococcus mutansen_US
dc.subjectA-tomatineen_US
dc.titleThe impact of a-tomatine on shear bonding strength in different dentin types and on cariogenic microorganisms: an in vitro and in silico study
dc.typeArticle

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