Endometrial injury concurrent with hysteroscopy increases the expression of Leukaemia inhibitory factor: A preliminary study
Yükleniyor...
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Reproductive Biology and Endocrinology
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
It is not known by which mechanism endometrial injury increases pregnancy rates. Leukaemia inhibitory factor (LIF) is a cytokine involved in wound healing and implantation. The aim of this study was to determine the
change in endometrial LIF mRNA expression before and after mechanical injury during hysteroscopy.
Methods: Forty patients with a history of two or more unsuccessful implantations who decided to undergo hysteroscopy in the proliferative phase were divided into two equal groups: one with endometrial injury (scratching group)
and the other with noninjury (control group). Endometrial sampling was conducted before injury on the patients in
the scratching group, and then injury was performed with monopolar needle forceps. Only diagnostic hysteroscopy
was performed on the patients in the control group. Endometrial tissues were collected using a Pipelle catheter
between Days 20 and 23 of the mid-luteal phase of the next cycles in both the scratching and control groups. Endometrial LIF mRNA expression was evaluated with the use of reverse-transcription polymerase chain reactions.
Results: Relative changes in mRNA expression levels of the LIF gene in endometrial samples taken before and after
injury were calculated using the 2-ΔΔCt method, and the fold changes obtained were compared between and within
the groups. Compared with preinjury values, an 11.1-fold increase was found in postinjury LIF mRNA expression in
patients with monopolar forceps injury (p < 0.001). There was a 3.9-fold signifcant increase in postinjury LIF mRNA
levels compared with those in the control group (p < 0.02).
Conclusions: The fertility-promoting efect of hysteroscopy-guided mechanical endometrial injury may be mediated
by LIF mRNA.
Açıklama
Anahtar Kelimeler
Endometrial Injury, Hysteroscopy, Monopolar Forceps, LIF mRNA, RT–PCR
Kaynak
Reproductive Biology and Endocrinology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
20
Sayı
1
Künye
Ersahin, S. S., & Ersahin, A. (2022). Endometrial injury concurrent with hysteroscopy increases the expression of Leukaemia inhibitory factor: a preliminary study. Reproductive Biology and Endocrinology, 20(1), 1-6.
