Evaluating the persistence of laundered semen stains on fabric using a forensic light source system, prostate-specific antigen Semiquant test and DNA recovery-profiling
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Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sage Publications Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Semen stains on the clothes of victims of sexual assault can remain as evidence even after garments have been laundered. In this study, we aimed to investigate the effectiveness of commonly preferred methods to detect semen stains in two different fabric types that were laundered with different washing machine programmes and washing powders, and to obtain a DNA profile from the semen stains. For this purpose, a comprehensive study was performed on semen-stained underwear using three different methods for stain detection, confirmation and identification: a forensic light source (FLS) system, the prostate-specific antigen (PSA) test and DNA recovery profiling. With FLS applications, stronger fluorescence was achieved in wash protocols performed at a low temperature (30 degrees C) on semen-stained cotton underwear. DNA recovery between 13.45 and 55.00 ng/mu l was obtained by modifications in the DNA extraction step when the effect of temperature and washing powder on DNA recovery was evaluated, and these were enough for short tandem repeat (STR) typing in all samples. This study shows that when semen-stained underwear is washed after a month, some semen stains can be determined by FLS and PSA, and all stains can be identified by STR analyses.
Açıklama
KARADAYI, BEYTULLAH/0000-0002-1728-0550; Karadayi, Sukriye/0000-0002-4253-9245
Anahtar Kelimeler
Laundered Semen Stains, forensic Light Source, PSA Test, DNA Recovery, DNA Profiling, STR
Kaynak
Medicine Science and the Law
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
60
Sayı
2
