Evaluating the persistence of laundered semen stains on fabric using a forensic light source system, prostate-specific antigen Semiquant test and DNA recovery-profiling

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dc.contributor.authorKaradayı, Şükriye
dc.contributor.authorMoshfeghi, Elnaz
dc.contributor.authorArasoğlu, Tülin
dc.contributor.authorKaradayı, Beytullah
dc.date.accessioned2021-05-15T11:33:51Z
dc.date.available2021-05-15T11:33:51Z
dc.date.issued2020
dc.departmentMeslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokuluen_US
dc.descriptionKARADAYI, BEYTULLAH/0000-0002-1728-0550; Karadayi, Sukriye/0000-0002-4253-9245
dc.description.abstractSemen stains on the clothes of victims of sexual assault can remain as evidence even after garments have been laundered. In this study, we aimed to investigate the effectiveness of commonly preferred methods to detect semen stains in two different fabric types that were laundered with different washing machine programmes and washing powders, and to obtain a DNA profile from the semen stains. For this purpose, a comprehensive study was performed on semen-stained underwear using three different methods for stain detection, confirmation and identification: a forensic light source (FLS) system, the prostate-specific antigen (PSA) test and DNA recovery profiling. With FLS applications, stronger fluorescence was achieved in wash protocols performed at a low temperature (30 degrees C) on semen-stained cotton underwear. DNA recovery between 13.45 and 55.00 ng/mu l was obtained by modifications in the DNA extraction step when the effect of temperature and washing powder on DNA recovery was evaluated, and these were enough for short tandem repeat (STR) typing in all samples. This study shows that when semen-stained underwear is washed after a month, some semen stains can be determined by FLS and PSA, and all stains can be identified by STR analyses.en_US
dc.identifier.doi10.1177/0025802419896935
dc.identifier.endpage130en_US
dc.identifier.issn0025-8024
dc.identifier.issn2042-1818
dc.identifier.issue2en_US
dc.identifier.pmid32098574
dc.identifier.scopus2-s2.0-85081600097
dc.identifier.scopusqualityQ1
dc.identifier.startpage122en_US
dc.identifier.urihttps://doi.org/10.1177/0025802419896935
dc.identifier.urihttps://hdl.handle.net/20.500.12939/238
dc.identifier.volume60en_US
dc.identifier.wosWOS:000516693900001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaradayı, Şükriye
dc.language.isoen
dc.publisherSage Publications Incen_US
dc.relation.ispartofMedicine Science and the Law
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLaundered Semen Stainsen_US
dc.subjectforensic Light Sourceen_US
dc.subjectPSA Testen_US
dc.subjectDNA Recoveryen_US
dc.subjectDNA Profilingen_US
dc.subjectSTRen_US
dc.titleEvaluating the persistence of laundered semen stains on fabric using a forensic light source system, prostate-specific antigen Semiquant test and DNA recovery-profiling
dc.typeArticle

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WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Sağlık Hizmetleri Meslek Yüksekokulu Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu