Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates

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dc.contributor.authorBorsa, Barış Ata
dc.contributor.authorTuna, Bilge G.
dc.contributor.authorHernandez, Frank J.
dc.contributor.authorHernandez, Luiza I.
dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorArıca, M. Yakup
dc.contributor.authorÖzalp, Veli Cengiz
dc.date.accessioned2021-05-15T12:36:54Z
dc.date.available2021-05-15T12:36:54Z
dc.date.issued2016
dc.departmentTıp Fakültesi, Biyokimya Anabilim Dalıen_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990; Tuna, Bilge Guvenc/0000-0003-1348-1336; Hernandez, Frank J./0000-0001-6308-8087
dc.description.abstractA fast, specific and sensitive homogeneous assay for Staphylococcus aureus detection was developed by measuring the activity of secreted nuclease from the bacteria via a modified DNA oligonucleotide. As biosensor format, an effective system, Nanokeepers as previously reported, were used for triggered release of confined fluorophores, and hence specific detection of S. aureus on nuclease activity was obtained. The interference from blood components for fluorescent quantification was eliminated by a pre purification by aptamer-functionalized silica magnetic nanoparticles. The reported assay system was exclusively formed by nucleic acid oligos and magnetic or mesoporous silica nanoparticles, that can be used on blood samples in a stepwise manner. The assay was successfully used as a sensing platform for the specific detection of S. aureus cells as low as 682 CFU in whole blood. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK 1001 projectTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [213M315]en_US
dc.description.sponsorshipThis work was partially supported by TUBITAK 1001 project no: 213M315.en_US
dc.identifier.doi10.1016/j.bios.2016.06.023
dc.identifier.endpage32en_US
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid27318566
dc.identifier.scopus2-s2.0-84974718085
dc.identifier.scopusqualityQ1
dc.identifier.startpage27en_US
dc.identifier.urihttps://doi.org/10.1016/j.bios.2016.06.023
dc.identifier.urihttps://hdl.handle.net/20.500.12939/432
dc.identifier.volume86en_US
dc.identifier.wosWOS:000384853300005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBorsa, Barış Ata
dc.institutionauthorÖzalp, Veli Cengiz
dc.language.isoen
dc.publisherElsevier Advanced Technologyen_US
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicrococcal Nucleaseen_US
dc.subjectStaphylococcus Aureusen_US
dc.subjectAptamersen_US
dc.subjectNanoparticlesen_US
dc.subjectMagnetic Separationen_US
dc.titleStaphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates
dc.typeArticle

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