Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

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dc.contributor.authorŞişmanoğlu, Soner
dc.contributor.authorDemirci, Mustafa
dc.contributor.authorSchweikl, Helmut
dc.contributor.authorÖzen-Eroğlu, Güneş
dc.contributor.authorÇetin-Aktaş, Esin
dc.contributor.authorKuruca, Serap
dc.contributor.authorTekce, Neslihan
dc.date.accessioned2021-05-15T11:33:44Z
dc.date.available2021-05-15T11:33:44Z
dc.date.issued2020
dc.departmentDiş Hekimliği Fakültesi, Restoratif Diş Hekimliği Anabilim Dalıen_US
dc.descriptionSismanoglu, Soner/0000-0002-1272-5581; Tekce, Neslihan/0000-0002-5447-3159; erdem kuruca, serap/0000-0001-7878-9994; Demirci, Mustafa/0000-0002-9297-6945
dc.description.abstractPURPOSE. The effects of four different self-adhesive resin cement materials on cell viability and apoptosis after direct and indirect exposure were evaluated using different cell culture techniques. MATERIALS AND METHODS. Self-adhesive cements were applied to NIH/3T3 mouse fibroblasts by the extract test method, cell culture inserts, and dentin barrier test method. After exposure periods of 24 h and 72 h, the cytotoxicity of these self-adhesive materials was evaluated using the MTT assay (viability) and the Annexin-V-FITC/P1 staining (apoptosis). RESULTS. The lowest cell viability was found in cells exposed to BeautiCem SA for 24 h in the extract test method. Cell viability was reduced to 70.6% compared to negative controls. After the 72 h exposure period, viability rate of cell cultures exposed to BeautiCem SA decreased more than 2- fold (29.5%) while cells exposed to RelyX U200 showed the highest viability rate of 71.4%. In the dentin barrier test method, BeautiCem SA induced the highest number of cells in apoptosis after a 24 h exposure (4.1%). Panavia SA Cement Plus was the material that caused the lowest number of cells in apoptosis (1.5%). CONCLUSION. The used self-adhesive cements have showed different cytotoxic effects based on the evaluation method. As exposure time increased, the materials showed more cytotoxic and apoptotic effects. BeautiCem SA caused significantly more severe cytotoxic and apoptotic effects than other cements tested. Moreover, cements other than BeautiCem SA have caused necrotic cell death rather than apoptotic cell death.en_US
dc.description.sponsorshipResearch Fund of Istanbul UniversityIstanbul University [23665]en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Istanbul University. Project No. 23665.en_US
dc.identifier.doi10.4047/jap.2020.12.2.89
dc.identifier.endpage99en_US
dc.identifier.issn2005-7806
dc.identifier.issn2005-7814
dc.identifier.issue2en_US
dc.identifier.pmid32377322
dc.identifier.scopus2-s2.0-85084752852
dc.identifier.scopusqualityQ1
dc.identifier.startpage89en_US
dc.identifier.urihttps://doi.org/10.4047/jap.2020.12.2.89
dc.identifier.urihttps://hdl.handle.net/20.500.12939/220
dc.identifier.volume12en_US
dc.identifier.wosWOS:000529831000007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorŞişmanoğlu, Soner
dc.language.isoen
dc.publisherKorean Acad Prosthodonticsen_US
dc.relation.ispartofJournal of Advanced Prosthodontics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectBiocompatibilityen_US
dc.subjectCell Cultureen_US
dc.subjectCytotoxicityen_US
dc.subjectSelf-Adhesive Resin Cementsen_US
dc.titleCytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis
dc.typeArticle

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