Effects of calcium silicate-based cements on odonto/ osteogenic differentiation potential in mesenchymal stem cells
[ X ]
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Australian Endodontic Journal
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The objective of this study was to evaluate the biological effects and odonto/osteogenic differentiation potential of Biodentine, NeoMTA Plus and TheraCal LC in tooth germ-derived stem cells(TGSCs). TGSCs were exposed to the material extracts. Biocompatibility was tested with MTS cell proliferation assay. Odonto/osteogenic differentiation was assessed with alkaline phosphatase (ALP) activity and mRNA gene expressions (RUNX2, DSPP and DMP-1). Scanning electronic microscopy/ energy-dispersive X-ray (SEM/EDX) analysis and pH analysis were also performed for the materials. Data were evaluated using the one-way ANOVA and Tukey's tests. TGSCs remained viable after 7 days of incubation with all tested materials. Biodentine and NeoMTA Plus showed high ALP activity and increased expression of RUNX2, DSPP and DMP-1 compared to that of TheraCal LC. All materials can induce odonto/osteogenic differentiation of MSCs in various levels. Biocompatibility and odonto/osteogenic differentiation potential of Biodentine and NeoMTA Plus are similar and superior to that of TheraCal LC.
Açıklama
Anahtar Kelimeler
Calcium Silicates, Mesenchymal Stem Cells, Mesenchymal Stem Cells, Odonto/Osteogenic Differentiation, Pulp Capping, Vital Pulp Therapy
Kaynak
Australian Endodontic Journal
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
49
Sayı
1
Künye
Sismanoglu, S., & Ercal, P. (2022). Effects of calcium silicate‐based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells. Australian Endodontic Journal.
