Fourier transform infrared imaging—A novel approach to monitor bio molecular changes in subacute mild traumatic brain injury

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dc.authorid0000-0002-7844-6876en_US
dc.contributor.authorRakib, Fazle
dc.contributor.authorAl-Saad, Khalid
dc.contributor.authorGarip Ustaoğlu, Şebnem
dc.contributor.authorUllah, Ehsan
dc.contributor.authorMall, Raghvendra
dc.contributor.authorThompson, Richard
dc.contributor.authorAbdelalim, Essam M.
dc.contributor.authorAhmed, Tariq
dc.contributor.authorSevercan, Feride
dc.contributor.authorAli, Mohamed H. M.
dc.date.accessioned2022-01-17T06:52:22Z
dc.date.available2022-01-17T06:52:22Z
dc.date.issued2021en_US
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.description.abstractTraumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution.en_US
dc.identifier.citationRakib, F., Al-Saad, K., Ustaoglu, S. G., Ullah, E., Mall, R., Thompson, R., ... & Ali, M. H. (2021). Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury. Brain Sciences, 11(7), 918.en_US
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-85111054499
dc.identifier.scopusqualityQ3
dc.identifier.startpage918en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12939/2187
dc.identifier.volume11en_US
dc.identifier.wosWOS:000676737600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGarip Ustaoğlu, Şebnem
dc.institutionauthorSevercan, Feride
dc.language.isoen
dc.publisherMDPIen_US
dc.relation.ispartofBrain Sciences
dc.relation.isversionof10.3390/brainsci11070918en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFourier Transform Infrareden_US
dc.subjectTraumatic brain injuryen_US
dc.subjectBiochemical Analysis and Immunohistochemistryen_US
dc.titleFourier transform infrared imaging—A novel approach to monitor bio molecular changes in subacute mild traumatic brain injury
dc.typeArticle

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