Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: Optimisation and cellular studies
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Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Nasal immunisation with nanoparticles has already shown promising results. In this study, nanoparticle composites carrying BSA for nasal vaccination prepared using electrostatic interaction process between polycation N-trimethyl chitosan chloride (TMC), chitosan glutamate (CG), chitosan chloride (CCl) and polyanion carboxymethyl pullulan (CMP). A mass ratio of 2:1 for TMC-CMP combination produced stable nanocarriers. For CCl-CMP and CG-CMP formulations needed a mass ratio of 3:1. Loading efficiency was >90% for all formulations. Nanoparticles' size ranged from 207 to 603 nm. The surface charge of the complexes varied between +14 and +33 mV. SDS-PAGE integrity of the model antigen was also demonstrated. MTT studies showed that nanoparticle composites were less toxic to Calu-3 cells than the particles of cationic polymers alone. FITC-BSA loaded nanoparticles efficiently taken up by J774A.1 macrophages as confirmed by confocal microscopy highlighting the potential of these novel nanoparticulate carriers' use for nasal vaccination.
Açıklama
Cevher, Erdal/0000-0002-0486-2252;
Anahtar Kelimeler
Carboxymethyl Pullulan, Cellular Uptake, Chitosan Derivatives, Nanoparticles, Nasal Vaccination, N-Trimethyl Chitosan Chloride, Polyion Complexation
Kaynak
Journal of Microencapsulation
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
32
Sayı
8
