Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: Optimisation and cellular studies

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dc.contributor.authorCevher, Erdal
dc.contributor.authorSalomon, Stefan K.
dc.contributor.authorMakrakis, Apostolos
dc.contributor.authorLi, Xiong Wei
dc.contributor.authorBrocchini, Steve
dc.contributor.authorAlpar, H. Oya
dc.date.accessioned2021-05-15T12:40:39Z
dc.date.available2021-05-15T12:40:39Z
dc.date.issued2015
dc.departmentEczacılık Fakültesien_US
dc.descriptionCevher, Erdal/0000-0002-0486-2252;
dc.description.abstractNasal immunisation with nanoparticles has already shown promising results. In this study, nanoparticle composites carrying BSA for nasal vaccination prepared using electrostatic interaction process between polycation N-trimethyl chitosan chloride (TMC), chitosan glutamate (CG), chitosan chloride (CCl) and polyanion carboxymethyl pullulan (CMP). A mass ratio of 2:1 for TMC-CMP combination produced stable nanocarriers. For CCl-CMP and CG-CMP formulations needed a mass ratio of 3:1. Loading efficiency was >90% for all formulations. Nanoparticles' size ranged from 207 to 603 nm. The surface charge of the complexes varied between +14 and +33 mV. SDS-PAGE integrity of the model antigen was also demonstrated. MTT studies showed that nanoparticle composites were less toxic to Calu-3 cells than the particles of cationic polymers alone. FITC-BSA loaded nanoparticles efficiently taken up by J774A.1 macrophages as confirmed by confocal microscopy highlighting the potential of these novel nanoparticulate carriers' use for nasal vaccination.en_US
dc.identifier.doi10.3109/02652048.2015.1073392
dc.identifier.endpage768en_US
dc.identifier.issn0265-2048
dc.identifier.issn1464-5246
dc.identifier.issue8en_US
dc.identifier.pmid26480961
dc.identifier.scopus2-s2.0-84945471005
dc.identifier.scopusqualityQ2
dc.identifier.startpage755en_US
dc.identifier.urihttps://doi.org/10.3109/02652048.2015.1073392
dc.identifier.urihttps://hdl.handle.net/20.500.12939/645
dc.identifier.volume32en_US
dc.identifier.wosWOS:000369970100004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAlpar, H. Oya
dc.language.isoen
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal of Microencapsulation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarboxymethyl Pullulanen_US
dc.subjectCellular Uptakeen_US
dc.subjectChitosan Derivativesen_US
dc.subjectNanoparticlesen_US
dc.subjectNasal Vaccinationen_US
dc.subjectN-Trimethyl Chitosan Chlorideen_US
dc.subjectPolyion Complexationen_US
dc.titleDevelopment of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: Optimisation and cellular studies
dc.typeArticle

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