Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules
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Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Lateral flow assay (LFA) type of biosensors have been popular due to cost-effectiveness and easy-interpretation for instant results, most common examples of applications being pregnancy tests, food safety or medical diagnostics. There are several examples of reports with high sensitivity, including pre-concentration of the sample by magnetic pull-down. However, sensitivity and direct detection designs with aptamers has been a limiting factor for developing aptamers-based LFA assays. In this study, we report a lateral flow design based on aptamer-gated silica nanoparticles to develop high sensitivity and direct bacterial assay by shifting aptamers-target interaction to conjugation pad. Aptamer-gated silica nanoparticles-based biosensors were reported for their high sensitivity, specificity and label-free detection for small molecules and whole cells. This label-free strategy for LFA can determine L. monocytogenes in minced chicken matrix at less than 5 min with a limit of detection (LOD) of 53 cells in one mL samples.
Açıklama
Ozalp, Veli Cengiz/0000-0002-7659-5990; Kavruk, Murat/0000-0001-5331-7253; UCAK, SAMET/0000-0002-3461-2481
Anahtar Kelimeler
Aptamers, Listeria, Silica, Nanoparticles, Peroxidase
Kaynak
Analytical Biochemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
587
