Label-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules

Basit Öğe Kaydı
dc.contributor.authorTaşbaşı, B. Büşra
dc.contributor.authorGüner, Buket C.
dc.contributor.authorSudağıdan, Mert
dc.contributor.authorUçak, Samet
dc.contributor.authorKavruk, Murat
dc.contributor.authorÖzalp, Veli Cengiz
dc.date.accessioned2021-05-15T11:34:09Z
dc.date.available2021-05-15T11:34:09Z
dc.date.issued2019
dc.departmentTıp Fakültesi, Tıbbi Biyoloji Anabilim Dalıen_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990; Kavruk, Murat/0000-0001-5331-7253; UCAK, SAMET/0000-0002-3461-2481
dc.description.abstractLateral flow assay (LFA) type of biosensors have been popular due to cost-effectiveness and easy-interpretation for instant results, most common examples of applications being pregnancy tests, food safety or medical diagnostics. There are several examples of reports with high sensitivity, including pre-concentration of the sample by magnetic pull-down. However, sensitivity and direct detection designs with aptamers has been a limiting factor for developing aptamers-based LFA assays. In this study, we report a lateral flow design based on aptamer-gated silica nanoparticles to develop high sensitivity and direct bacterial assay by shifting aptamers-target interaction to conjugation pad. Aptamer-gated silica nanoparticles-based biosensors were reported for their high sensitivity, specificity and label-free detection for small molecules and whole cells. This label-free strategy for LFA can determine L. monocytogenes in minced chicken matrix at less than 5 min with a limit of detection (LOD) of 53 cells in one mL samples.en_US
dc.identifier.doi10.1016/j.ab.2019.113449
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.pmid31557463
dc.identifier.scopus2-s2.0-85072607305
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.ab.2019.113449
dc.identifier.urihttps://hdl.handle.net/20.500.12939/285
dc.identifier.volume587en_US
dc.identifier.wosWOS:000518177200009
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorUçak, Samet
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofAnalytical Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAptamersen_US
dc.subjectListeriaen_US
dc.subjectSilicaen_US
dc.subjectNanoparticlesen_US
dc.subjectPeroxidaseen_US
dc.titleLabel-free lateral flow assay for Listeria monocytogenes by aptamer-gated release of signal molecules
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Temel Tıp Bilimleri Bölümü Koleksiyonu