Theranostic potential of a novel aptamer specifically targeting HER2 in breast cancer cells

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dc.contributor.authorKüçükcankurt, Fulya
dc.contributor.authorUçak, Samet
dc.contributor.authorAltiok, Nedret
dc.date.accessioned2024-03-30T08:52:56Z
dc.date.available2024-03-30T08:52:56Z
dc.date.issued2024en_US
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri, Tıbbi Biyoloji Ana Bilim Dalıen_US
dc.description.abstractBackground/aim: The overexpression of HER2 is correlated with poorer outcomes and therapeutic resistance in breast cancer patients. While HER2-targeted therapies have shown improvement, prognosis remains poor for HER2-positive breast cancer patients, and these treatments have limitations. Therefore, it is crucial to explore effective molecular strategies for early detection and treatment of HER2-positive breast cancers. Materials and methods: In this study, we employed the cell-SELEX method to generate a selective aptamer capable of recognizing HER2 in its native conformation within breast cancer cells, for theranostic applications. Utilizing an adherent cell-SELEX approach, we developed and explored a DNA aptamer, named HMAP7, which can specifically target HER2 in the MDA-MB-453 and SK-BR-3 human breast cancer cell lines. After sequencing, the binding affinities of 10 candidate aptamers to HER2 receptors were evaluated by measuring fluorescence intensities within intact cells using near-infrared optical imaging. The dissociation constant of HMAP7 was determined to be in the nanomolar range in both cell lines. Results: The cell-SELEX-derived aptamer sequence, HMAP7 (41-mer), exhibited the highest binding affinity and specificity for HER2. HMAP7 was rapidly internalized into breast cancer cells overexpressing HER2 but showed no uptake in the HER2 receptor-deficient breast cancer cell line MDA-MB-231. Moreover, HMAP7 demonstrated remarkable selectivity for HER2, rendering it suitable for use in complex biological systems. Conclusions: Our findings suggest that the novel DNA aptamer HMAP7 holds promise for both therapeutic and diagnostic applications, enabling selective delivery of therapeutic agents or imaging of HER2-positive breast tumors.en_US
dc.identifier.citationKüçükcankurt, F., Uçak, S., Altiok, N. (2024). Theranostic potential of a novel aptamer specifically targeting HER2 in breast cancer cells. Turkish Journal of Biology, 48(1), 35-45. 10.55730/1300-0152.2680en_US
dc.identifier.endpage45en_US
dc.identifier.issn1300-0152
dc.identifier.issue1en_US
dc.identifier.pmid38665781
dc.identifier.scopusqualityQ1
dc.identifier.startpage35en_US
dc.identifier.trdizinid1239502
dc.identifier.urihttps://hdl.handle.net/20.500.12939/4646
dc.identifier.volume48en_US
dc.identifier.wosWOS:001178934500002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.institutionauthorKüçükcankurt, Fulya
dc.language.isoen
dc.publisherTübitaken_US
dc.relation.ispartofTurkish Journal of Biology
dc.relation.isversionof10.55730/1300-0152.2680en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak114S517
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAptameren_US
dc.subjectBreast canceren_US
dc.subjectCell-SELEXen_US
dc.subjectHER2en_US
dc.subjectOptical imagingen_US
dc.subjectTargeted therapyen_US
dc.titleTheranostic potential of a novel aptamer specifically targeting HER2 in breast cancer cells
dc.typeArticle

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