A facile and efficient method of enzyme immobilization on silica particles via Michael acceptor film coatings: immobilized catalase in a plug flow reactor
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Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel method was developed for facile immobilization of enzymes on silica surfaces. Herein, we describe a single-step strategy for generating of reactive double bonds capable of Michael addition on the surfaces of silica particles. This method was based on reactive thin film generation on the surfaces by heating of impregnated self-curable polymer, alpha-morpholine substituted poly(vinyl methyl ketone) p(VMK). The generated double bonds were demonstrated to be an efficient way for rapid incorporation of enzymes via Michael addition. Catalase was used as model enzyme in order to test the effect of immobilization methodology by the reactive film surface through Michael addition reaction. Finally, a plug flow type immobilized enzyme reactor was employed to estimate decomposition rate of hydrogen peroxide. The highly stable enzyme reactor could operate continuously for 120 h at 30 degrees C with only a loss of about 36 % of its initial activity.
Açıklama
Ozalp, Veli Cengiz/0000-0002-7659-5990; ince, ahmet/0000-0002-4111-1327
Anahtar Kelimeler
Michael Addition, Surface Modification, Catalase, Immobilized Enzymes, Enzyme Bioreactors
Kaynak
Bioprocess and Biosystems Engineering
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
39
Sayı
6
