A facile and efficient method of enzyme immobilization on silica particles via Michael acceptor film coatings: immobilized catalase in a plug flow reactor

Basit Öğe Kaydı
dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorArıca, M. Yakup
dc.contributor.authorGenç, Ayşenur
dc.contributor.authorÖzalp, V. Cengiz
dc.contributor.authorİnce, Ahmet
dc.contributor.authorBıçak, Niyazi
dc.date.accessioned2021-05-15T12:37:08Z
dc.date.available2021-05-15T12:37:08Z
dc.date.issued2016
dc.departmentTıp Fakültesien_US
dc.descriptionOzalp, Veli Cengiz/0000-0002-7659-5990; ince, ahmet/0000-0002-4111-1327
dc.description.abstractA novel method was developed for facile immobilization of enzymes on silica surfaces. Herein, we describe a single-step strategy for generating of reactive double bonds capable of Michael addition on the surfaces of silica particles. This method was based on reactive thin film generation on the surfaces by heating of impregnated self-curable polymer, alpha-morpholine substituted poly(vinyl methyl ketone) p(VMK). The generated double bonds were demonstrated to be an efficient way for rapid incorporation of enzymes via Michael addition. Catalase was used as model enzyme in order to test the effect of immobilization methodology by the reactive film surface through Michael addition reaction. Finally, a plug flow type immobilized enzyme reactor was employed to estimate decomposition rate of hydrogen peroxide. The highly stable enzyme reactor could operate continuously for 120 h at 30 degrees C with only a loss of about 36 % of its initial activity.en_US
dc.identifier.doi10.1007/s00449-016-1566-0
dc.identifier.endpage881en_US
dc.identifier.issn1615-7591
dc.identifier.issn1615-7605
dc.identifier.issue6en_US
dc.identifier.pmid26879642
dc.identifier.scopus2-s2.0-84971543384
dc.identifier.scopusqualityQ1
dc.identifier.startpage871en_US
dc.identifier.urihttps://doi.org/10.1007/s00449-016-1566-0
dc.identifier.urihttps://hdl.handle.net/20.500.12939/482
dc.identifier.volume39en_US
dc.identifier.wosWOS:000376486400002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzalp, V. Cengiz
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofBioprocess and Biosystems Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMichael Additionen_US
dc.subjectSurface Modificationen_US
dc.subjectCatalaseen_US
dc.subjectImmobilized Enzymesen_US
dc.subjectEnzyme Bioreactorsen_US
dc.titleA facile and efficient method of enzyme immobilization on silica particles via Michael acceptor film coatings: immobilized catalase in a plug flow reactor
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Temel Tıp Bilimleri Bölümü Koleksiyonu