Similar bacterial signatures in the gut microbiota of type 1 and type 2 diabetes patients and its association with G protein-coupled receptor 41 and 43 gene expression
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Science and Business Media Deutschland GmbH
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: There are conficting reports regarding the abundance of short-chain fatty acids producing bacteria in the gut
microbiota in patients with type 1 and type 2 diabetes. We aimed to determine the amount of Akkermansia muciniphila,
Anaerobutyricum hallii, Bifdobacterium adolescentis, Bifdobacterium longum, Collinsella aerofaciens, Faecalibacterium
prausnitzii, Lacticaseibacillus rhamnosus, and Parabacteroides distasonis in the gut microbiota in patients with type1 and
type2 diabetes, compared with the healthy controls and analyze the correlation between the gene expression levels of two
short-chain fatty acids receptors GPR41 and GPR43.
Methods: Forty type 1, 40 type 2 stool and blood samples of diabetes patients, and 40 healthy control samples were studied.
DNA and RNA were extracted, and bacteria were detected using a Microbial DNA qPCR Assay kit. Gene expressions were
detected with GPR41 and GPR43 primers via in-house qPCR.
Results: Compared with healthy controls, B.longum and F.prausnitzii abundance were signifcantly decreased in patients
with type1 and type2 diabetes, A.hallii abundance was increased in patients with type1 and decreased in type2 diabetes
contrarily A.muciniphila abundance was decreased in patients with type1 and increased in type2 diabetes. GPR43 gene
expression was upregulated in both patients group, however GPR41 was upregulated only in patients with type2 diabetes.
Conclusions: Elevated B. longum and F. prausnitzii abundances were detected in the gut microbiota of patients with type1
and type2 diabetes and compared with healthy controls. B. longum and F.prausnitzii abundances were also correlated with
the GPR43 gene expression level in type1 diabetes patients. Extensive studies determining bacteria producing short-chain
fatty acids in gut microbiota, and their contribution in the pathogenesis of diabetes, are needed to understand better the
mechanism of these diseases.
Açıklama
Anahtar Kelimeler
Type 1 Diabetes, Type 2 Diabetes, Gut Microbiota, Scfas Receptor
Kaynak
Journal of Diabetes and Metabolic Disorders
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
Sayı
Künye
Demirci, M., Taner, Z., Keskin, F. E., Ozyazar, M., Kiraz, N., Kocazeybek, B. S., Tokman, H. B. (2022). Similar bacterial signatures in the gut microbiota of type 1 and type 2 diabetes patients and its association with G protein-coupled receptor 41 and 43 gene expression. Journal of Diabetes and Metabolic Disorders.
