Similar bacterial signatures in the gut microbiota of type 1 and type 2 diabetes patients and its association with G protein-coupled receptor 41 and 43 gene expression

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dc.contributor.authorDemirci, Mehmet
dc.contributor.authorTaner, Zeynep
dc.contributor.authorKeskin, Fatma E.
dc.contributor.authorOzyazar, Mucahit
dc.contributor.authorKiraz, Nuri
dc.contributor.authorKocazeybek, Bekir S.
dc.contributor.authorTokman, Hrisi Bahar
dc.date.accessioned2022-07-18T09:21:34Z
dc.date.available2022-07-18T09:21:34Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri, Tıbbi Mikrobiyoloji Ana Bilim Dalıen_US
dc.description.abstractPurpose: There are conficting reports regarding the abundance of short-chain fatty acids producing bacteria in the gut microbiota in patients with type 1 and type 2 diabetes. We aimed to determine the amount of Akkermansia muciniphila, Anaerobutyricum hallii, Bifdobacterium adolescentis, Bifdobacterium longum, Collinsella aerofaciens, Faecalibacterium prausnitzii, Lacticaseibacillus rhamnosus, and Parabacteroides distasonis in the gut microbiota in patients with type1 and type2 diabetes, compared with the healthy controls and analyze the correlation between the gene expression levels of two short-chain fatty acids receptors GPR41 and GPR43. Methods: Forty type 1, 40 type 2 stool and blood samples of diabetes patients, and 40 healthy control samples were studied. DNA and RNA were extracted, and bacteria were detected using a Microbial DNA qPCR Assay kit. Gene expressions were detected with GPR41 and GPR43 primers via in-house qPCR. Results: Compared with healthy controls, B.longum and F.prausnitzii abundance were signifcantly decreased in patients with type1 and type2 diabetes, A.hallii abundance was increased in patients with type1 and decreased in type2 diabetes contrarily A.muciniphila abundance was decreased in patients with type1 and increased in type2 diabetes. GPR43 gene expression was upregulated in both patients group, however GPR41 was upregulated only in patients with type2 diabetes. Conclusions: Elevated B. longum and F. prausnitzii abundances were detected in the gut microbiota of patients with type1 and type2 diabetes and compared with healthy controls. B. longum and F.prausnitzii abundances were also correlated with the GPR43 gene expression level in type1 diabetes patients. Extensive studies determining bacteria producing short-chain fatty acids in gut microbiota, and their contribution in the pathogenesis of diabetes, are needed to understand better the mechanism of these diseases.en_US
dc.identifier.citationDemirci, M., Taner, Z., Keskin, F. E., Ozyazar, M., Kiraz, N., Kocazeybek, B. S., Tokman, H. B. (2022). Similar bacterial signatures in the gut microbiota of type 1 and type 2 diabetes patients and its association with G protein-coupled receptor 41 and 43 gene expression. Journal of Diabetes and Metabolic Disorders.en_US
dc.identifier.issn22516581
dc.identifier.scopus2-s2.0-85133201360
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://hdl.handle.net/20.500.12939/2669
dc.identifier.wosWOS:000819309300001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTaner, Zeynep
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbHen_US
dc.relation.ispartofJournal of Diabetes and Metabolic Disorders
dc.relation.isversionof10.1007/s40200-022-01068-2en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectType 1 Diabetesen_US
dc.subjectType 2 Diabetesen_US
dc.subjectGut Microbiotaen_US
dc.subjectScfas Receptoren_US
dc.titleSimilar bacterial signatures in the gut microbiota of type 1 and type 2 diabetes patients and its association with G protein-coupled receptor 41 and 43 gene expression
dc.typeArticle

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