Diagnosis of malignant pleural mesothelioma from pleural fluid by Fourier transform-infrared spectroscopy coupled with chemometrics
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Spie-Soc Photo-Optical Instrumentation Engineers
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study was conducted to differentiate malignant pleural mesothelioma (MPM) from lung cancer (LC) and benign pleural effusion (BPE) from pleural fluids using the diagnostic power of Fourier transform-infrared spectroscopy with attenuated total reflectance mode coupled with chemometrics. Infrared spectra of MPM (n = 24), LC (n = 20), and BPE (n = 25) were collected, and hierarchical cluster analysis (HCA) and principal component analysis (PCA) were applied to their spectra. HCA results indicated that MPM was differentiated from LC with 100% sensitivity and 100% specificity and from BPE, with 100% sensitivity and 88% specificity, which were also confirmed by PCA score plots. PCA loading plots indicated that these separations originated mainly from lipids, proteins, and nucleic acids-related spectral bands. There was significantly higher lipid, protein, nucleic acid, and glucose contents in the MPM and LC. However, the significant changes in triglyceride and cholesterol ester content, protein and nucleic acid structure, a lower membrane fluidity, and higher membrane order were only observed in the MPM. To check the classification success of some test samples/each group, soft independent modeling of class analogies was performed and 96.2% overall classification success was obtained. This approach can provide a rapid and inexpensive methodology for the efficient differentiation of MPM from other pleural effusions. (C) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Açıklama
Abbas, Sherif/0000-0001-8631-5027; Koksal, Deniz/0000-0001-8374-3691
Anahtar Kelimeler
PleuralFluid, Malignant Pleural Mesothelioma, Lung Cancer, Fourier Transform-Infrared Spectroscopy, Chemometrics, Benign Pleural Effusion
Kaynak
Journal of Biomedical Optics
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
23
Sayı
10
